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Separation of cysteine derivatives by HPLC with electrochemical detection
Summary
Type:
HPLC
Application #:
107650
Catalog#:
720182.46 - MN HPLC Column, Nucleosil SA, 100A, 5um, 4.6x150mm
Detection:
electrochemical, working electrode Au, + 800mV
Conditions:
eluent: H2O with 4.5g/l ammonium citrate and 6g/l phosphoric acid pH 2.2
flow rate 1ml/min
Authors:
Metrohm
Source:
Metrohm Application Bulletin 128 d (1980) 26 27
Details
Legend: Left: Chromatogram of 1. L-cysteine, 2. L-glutathione, 3. penicillamine (20ng each injected) right: penicillamine from urine diluted with eluent (20µg/ml, 20µl injected).
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